FastQCFastQC Report
Mon 6 Jun 2016
ERR1378483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035250
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCC26440.25539724704177735Illumina Paired End PCR Primer 2 (97% over 38bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCCGTC25540.24670369475971987Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT14290.13803429123400143Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3300.037.5042743
ATGCCGT3750.033.00375744
TGCCGTC3750.033.00375745
ACGTCAG450.001227386124.99680736
CGGAAGA6900.023.500231
GATCGGA2500.023.3970126
ATCGGAA2550.022.93935627
ATCGTAG500.00226550422.49821334
CGTATGC6950.022.34067344
ACCGCTC6950.022.33635530
AGACCGC6950.022.33635528
TATCTCG6950.022.33527839
TCGTATG7100.021.86868743
CTCGTAT7100.021.86657342
GACCGCT7150.021.71156329
CGCTCTC7350.021.42686832
GAGACCG7250.021.4120927
TCTCGTA7400.020.97907641
CCGCTCT7400.020.97806431
TCGGAAG2900.020.94661328
Produced by FastQC (version 0.11.5)