FastQCFastQC Report
Mon 6 Jun 2016
ERR1378482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524713
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCCGTC33930.6466392103873927Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCC33430.6371101916666826Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18220.34723744218267893Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7950.15151139765929186Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAC207.032125E-444.99695225
TATGCCG4200.036.96882643
TGCCGTC4550.034.1250745
ATGCCGT4700.033.03597344
CCACCGT500.002261367222.5027647
CCGGTAG7350.021.4271228
ATCGGAA3150.021.4271227
CGTGAGC854.9934515E-621.191191
ACCGGTA7500.020.99857727
GACCGGT7500.020.99857726
CGGTAGC7500.020.99857729
TCGGAAG3650.020.95748328
CGGAAGA7000.020.585731
TCTCGTA7550.020.5615238
CGTATGC7700.020.4531641
GATCGGA3300.020.4531626
CGAGGTC550.003935275620.4531623
GTAGCAA7750.020.32120331
CTCGTAT7650.020.29274239
TCGTATG7650.020.29274240
Produced by FastQC (version 0.11.5)