FastQCFastQC Report
Mon 6 Jun 2016
ERR1378481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679043
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCCGTC39240.5778720935198507Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCC31370.46197368944234757Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT19140.2818672749737498Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8320.12252537762704277Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4400.039.8953143
TGCCGTC4600.038.1607345
ATGCCGT4750.036.9556544
ATCGGAA3250.028.38261827
GATCGGA3400.027.13044526
TCGGAAG3500.026.35528828
CCGAGAT3850.025.12810122
AGATCGG3700.024.93067725
CGTATGC7450.023.56059541
CTCGTAT7550.023.24339739
CCGATAG7750.023.22417328
ATAGCGA7600.023.0904831
CGATAGC7700.023.0827929
AATGCCG12000.023.06087718
TGCCGAG11950.022.96909520
TCTCGTA7650.022.93956238
ACCGATA7750.022.9338727
GACCGAT7800.022.78685826
GATAGCG7800.022.78685830
TCGTATG7750.022.64356840
Produced by FastQC (version 0.11.5)