FastQCFastQC Report
Mon 6 Jun 2016
ERR1378480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601255
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATACGATCTCGTATGCC27430.4562124223499181Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATACGATCTCGTATGCCGTC27400.45571346600028273Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT14070.2340105279789773Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTC207.033608E-444.9960915
ATGCCGT3800.039.6841144
TATGCCG3950.038.74692543
TGCCGTC4050.037.7902145
GTATGCC6050.025.66319742
CGTATGC6050.025.29126741
CTCGTAT6050.024.91519239
GATACGA6100.024.71096831
TCTCGTA6200.024.67527638
TACGATC6150.024.51006733
GGATACG6200.024.31240330
CGGATAC6200.024.31240329
AGACCGG6300.024.28360425
GACCGGA6300.023.9264926
CCGGATA6300.023.9264928
ATACGAT6300.023.9264932
TCGTATG6400.023.90417340
ATCTCGT6450.023.7188737
GAGACCG6450.023.7188724
ACCGGAT6450.023.37006227
Produced by FastQC (version 0.11.5)