FastQCFastQC Report
Mon 6 Jun 2016
ERR1378477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915423
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTAGATCTCGTATGCCGTC36830.40232766710034595Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTAGATCTCGTATGCC26390.28828202918213763Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT20770.22688964555183777Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4450.034.8906943
ATGCCGT4400.034.7757744
TGCCGTC4700.033.034845
CGCTATT300.00514864229.99770520
GATCGGA4600.026.41102426
ATCGGAA4700.025.850527
AGATCGG4750.025.57699225
TCGGAAG4950.024.99945328
CCGAGAT5450.022.70468722
TGCCGAG12550.022.58791420
CGGTGTA7000.022.4995129
AATGCCG12600.022.49827818
ATGCCGA12850.022.0605719
CCGGTGT7200.021.87452128
ACCGGTG7150.021.71281227
GACCGGT7250.021.41215526
TCGTATG7300.021.26665940
GAGATCG5750.021.12881924
CGTATGC7350.021.12198841
TCTCGTA7400.020.97812538
Produced by FastQC (version 0.11.5)