FastQCFastQC Report
Mon 6 Jun 2016
ERR1378476.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378476.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886097
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGTAGATCTCGTATGCC27830.3140739670713251Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACGTAGATCTCGTATGCCGTC23640.2667879475949021Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11970.13508679072381466Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3300.032.73162543
TGCCGTC3500.030.86124645
ATGCCGT3600.029.37890644
AGATCGG2150.025.11394925
ATCGGAA2250.023.99912827
GATCGGA2300.023.47740626
CGGAAGA5700.022.514431
TGAACCG702.7040276E-522.49791310
CGACGTA5650.021.50364132
ACCGACG5750.021.12966530
CCGACGT5800.020.94751531
GACGTAG5850.020.76847533
TCGGAAG2550.020.2933828
GACCGAC6000.020.24926229
ACGTAGA5900.020.2111334
AGACCGA6200.019.95895228
CGTATGC6000.019.87764244
TCGTATG6050.019.71336643
CGTAGAT6100.019.54957235
GTATGCC6350.019.4907845
Produced by FastQC (version 0.11.5)