FastQCFastQC Report
Mon 6 Jun 2016
ERR1378475.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378475.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942018
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACTAGATCTCGTATGCC27100.2876802778715481Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGACTAGATCTCGTATGCCGTC26970.28630026177843737Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13910.14766172196284996Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3400.038.38787543
ATGCCGT3550.037.3997544
TGCCGTC3700.035.27534545
CGTATGC5850.022.6967841
ACCGGAC5800.022.49845927
CGGACTA5800.022.49845929
ACGTCAT500.002265908522.49726720
TCTCGTA5850.022.3073538
TCGTATG5850.022.3073540
GACCGGA5850.022.30616626
CTCGTAT5900.022.11830539
CCGGACT5900.022.1171328
AGACCGG5900.022.11595525
ATCTCGT6100.021.39311637
GATCGGA2600.020.76780926
GGACTAG6450.020.57998730
GTATGCC6350.020.55304742
CGGAAGA5400.020.4337081
ATCGGAA2550.020.2927327
GAGACCG6500.020.07448424
Produced by FastQC (version 0.11.5)