FastQCFastQC Report
Mon 6 Jun 2016
ERR1378474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869641
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGACATCTCGTATGCC54730.6293401530056655Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGACATCTCGTATGCCGTC49210.5658656848055692Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT25040.2879349064729009Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10510.12085446753315449Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5650.037.4349744
TGCCGTC5800.036.4668245
TATGCCG5850.036.1551443
ATCGGAA4500.028.49774227
GATCGGA4450.028.31236326
AGATCGG4600.027.38913325
CGTATAA801.053977E-725.31049510
TCGGAAG5250.025.2837128
CGGAAGA11550.024.1747821
GTATGCC12350.022.591945
CGTATGC12500.022.32079744
GACCGAG12450.022.22715629
CCGAGAT5900.022.11689422
AGACCGA12550.022.05004528
CTCGTAT12600.021.96254542
GAGACCG12650.021.87573627
CGAGTGA12700.021.78961432
ACCGAGT12700.021.78961430
TCTCGTA12700.021.78961441
CATCTCG13000.021.63290239
Produced by FastQC (version 0.11.5)