FastQCFastQC Report
Mon 6 Jun 2016
ERR1378473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918115
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGACATCTCGTATGCCGTC54760.5964394438605186Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGACATCTCGTATGCC40250.4383982398719115Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT24520.2670689401654477Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC11920.1298312302925015Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5950.038.21145243
ATGCCGT6050.037.95400244
TGCCGTC5950.037.83518245
CGTATGC9300.024.67706141
CTCGTAT9300.024.67571839
TCGTATG9400.024.41320840
ATCTCGT9450.024.28403937
TCTCGTA9450.024.28403938
ACCGGAT9500.023.918127
CGGATGA9600.023.903329
GACCGGA9450.023.80658326
GTATGCC9850.023.75858742
AGACCGG9750.023.30481725
ATCGGAA4950.023.17895727
CATCTCG9950.023.06373636
CCGGATG9950.023.06247928
GGATGAC10250.023.04593530
GATCGGA5150.022.27880326
GAGACCG10300.022.06038324
TCGGAAG5600.021.69374828
Produced by FastQC (version 0.11.5)