FastQCFastQC Report
Mon 6 Jun 2016
ERR1378471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016225
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATCGAGATCTCGTATGCCGTC62650.6164973308076459Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCGAGATCTCGTATGCC34710.3415582179143398Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT29340.28871558955939874Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC15540.15291889099362838Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7600.039.6886343
ATGCCGT7700.039.1731944
TGCCGTC7700.039.1731945
GTACGTG300.005150696729.99557323
CGTATGC11650.026.0755341
CTCGTAT11700.025.76796339
TCGTATG11750.025.65831240
TCTCGTA11800.025.35642638
ACCGATC11950.025.04060727
GACCGAT12000.024.93381926
GTATGCC12100.024.92226242
AGACCGA12100.024.9136825
GAGACCG12150.024.81115324
ATCTCGT11800.024.78447737
CCGATCG12200.024.71189928
CGATCGA12400.024.31331829
TCGAGAT12550.023.84344732
ATCGAGA12950.023.28070631
AATGCCG18950.023.03090118
ATGCCGA19150.022.67289419
Produced by FastQC (version 0.11.5)