FastQCFastQC Report
Mon 6 Jun 2016
ERR1378469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798925
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGATATCTCGTATGCCGTC36440.45611290171167507Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGATATCTCGTATGCC25870.32381011984854646Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18520.23181149669868886Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.039.79722643
ATGCCGT4900.038.1196744
TGCCGTC5050.037.43303345
TCTCGTA6850.027.59143438
CGTATGC6950.027.19613641
CTCGTAT7000.027.00018739
TCGTATG7050.026.80869740
AGACCGG7100.026.61823725
GTATGCC7200.026.56434842
ACCGGCA7150.026.43209527
TATCTCG7300.025.89059336
GAGACCG7400.025.5391224
GATCGGA3450.025.43336726
ATCGGAA3450.025.43336727
AATGCCG11300.025.2846218
ATCTCGT7500.025.20017637
CCGGCAG7500.025.19859928
CGGCAGA7700.025.12847129
TCGGAAG3600.024.9986128
ATGCCGA11550.024.3477719
Produced by FastQC (version 0.11.5)