FastQCFastQC Report
Mon 6 Jun 2016
ERR1378468.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378468.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853474
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACAGATCTCGTATGCC28730.33662419710500846Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACAGATCTCGTATGCCGTC25530.2991303777267966Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12930.15149846392508734Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3500.039.22490743
TGCCGTC3550.036.7726845
ATGCCGT3750.034.81146644
TAAGCGG300.005150031829.99582115
ATCGGAA2650.025.46815127
AGATCGG2800.024.90724425
GATCGGA2750.024.54203426
CTCGTAT7300.023.11728142
CGTGACA7400.022.80354932
CGGAAGA6950.022.6786441
CGTATGC7350.022.66052444
TCTCGTA7350.022.65521441
AGACCGT7450.022.65050528
ATGCGCC702.6987324E-522.503462
TCGTATG7450.022.3550543
ATCTCGT7450.022.348540
TCGGAAG3050.022.13065728
CCGTGAC7600.021.9074131
GTATGCC7650.021.77187745
GAGACCG7750.021.48087927
Produced by FastQC (version 0.11.5)