FastQCFastQC Report
Mon 6 Jun 2016
ERR1378466.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378466.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844382
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTGTAATCTCGTATGCCGTC35570.4212548348970016Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTGTAATCTCGTATGCC33140.3924763910173357Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT17200.2036992735515442Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3850.036.8250645
TATGCCG3850.036.2405443
ATGCCGT4000.034.8815244
GATCGGA2950.026.69226
ATCGGAA2950.026.69227
AGATCGG3150.024.99726925
AACGATC551.3671475E-424.54713441
TCGGAAG3250.024.22955728
CGGAAGA6800.022.5175461
CACACGC754.594153E-521.00392343
AATGCCG10600.020.79961418
ATGCCGA10650.020.70196319
TCTCGTA7350.020.51160841
ACCGTCT7350.020.50917830
CTCGTAT7500.020.40260542
CGTCTGT7500.020.39898132
CCGTCTG7500.020.39898131
AGACCGT7300.020.34144828
TCGTATG7550.020.26868843
TGCCGAG10900.020.22714820
Produced by FastQC (version 0.11.5)