FastQCFastQC Report
Mon 6 Jun 2016
ERR1378465.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378465.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751617
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTCTAATCTCGTATGCCGTC42130.5605248417744676Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTCTAATCTCGTATGCC38380.5106324098576802Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT20730.2758053636360008Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9010.1198748830854012Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5000.037.8062644
TATGCCG5050.037.43194243
TGCCGTC5050.037.43194245
GATCGGA4100.024.1455226
ATCGGAA4200.023.57062727
TCGGAAG4550.023.24096928
AGATCGG4300.023.02247425
GGGCGAC802.9733583E-622.49923528
CGGAAGA8800.020.727451
CGTGTCT9450.020.71358129
TGCCGAG14700.020.35509520
CGTATGC9400.020.34505341
AATGCCG14750.020.28609518
CTCGTAT9450.020.23740839
ATGCCGA14800.020.21756219
CCGTGTC9600.020.15556328
TCTCGTA9500.020.13089638
AGACCGT9550.020.02549725
TCGTATG9550.020.02549740
ATCTCGT9600.019.6868337
Produced by FastQC (version 0.11.5)