FastQCFastQC Report
Mon 6 Jun 2016
ERR1378464.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378464.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767663
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCTGATCTCGTATGCCGTC55110.7178931380045671Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCTGATCTCGTATGCC39260.5114223298504682Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT22750.2963539990855362Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC11880.1547554069949965Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT7450.040.4814644
TATGCCG7600.039.68248443
TGCCGTC7800.038.66498645
CGTAACA300.00514879329.99691814
GACCGTA11300.026.4795826
CCGTATC11350.026.36464728
ACCGTAT11500.026.02075827
CGTATGC11600.025.9954841
TCTCGTA11550.025.90642738
CGTATCT11650.025.68572829
CTCGTAT11650.025.68405539
TCGTATG11750.025.65693740
AGACCGT11850.025.25056825
GTATGCC11950.025.23410642
CCCGTAA450.001226918324.9974312
GAGACCG12050.024.83147424
ATCTCGT12250.024.42606237
GTATCTG12800.023.37802530
CGAGACC12950.023.10573223
CCGAGAC13150.022.925422
Produced by FastQC (version 0.11.5)