FastQCFastQC Report
Mon 6 Jun 2016
ERR1378463.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378463.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207403
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCCGTC52500.43481753813763924Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCC30040.24879845420294633Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT27440.22726463326660612Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT7650.037.65187544
TGCCGTC7600.037.60349345
TATGCCG7800.037.504843
GACCGAT10600.027.3799126
CCGATAC10700.027.1240228
CGTATGC10900.026.62853841
TCTCGTA10900.026.62633338
TCGTATG10900.026.62633340
CGATACT10900.026.62633329
ACCGATA11000.026.58880427
ATCTCGT10950.026.50584837
CTCGTAT11050.026.26488939
GTATGCC11150.026.03148742
GATCGGA5300.025.89417626
ATCGGAA5300.025.89417627
AGACCGA11250.025.79795825
AGATCGG5450.025.59430525
GAGACCG11500.025.23713324
CATCTCG11750.024.89267736
GATACTC11900.024.57788730
Produced by FastQC (version 0.11.5)