FastQCFastQC Report
Mon 6 Jun 2016
ERR1378462.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378462.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963255
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACTAATCTCGTATGCCGTC33350.3462219246201681Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACTAATCTCGTATGCC25630.26607699934077683Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18530.19236858360454917Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5300.039.0598943
TGCCGTC5800.035.6926645
ATGCCGT5850.035.38759644
CGTATGC7750.026.7105441
ACCGTCA7700.026.58900627
GACCGTC7850.026.07958426
CTCGTAT7950.026.03722639
TCGTATG7950.026.03722640
CGTCACT7950.026.03587329
GTATGCC8100.025.8341742
CCGTCAC7950.025.75287428
TCTCGTA8050.025.71244638
ATCTCGT8100.025.55372837
ATGCCGA12050.025.01636119
AGACCGT8250.024.81383125
ATCGGAA3850.024.543727
GAGACCG8400.024.37072624
AATCTCG8550.024.20879636
AATGCCG12600.023.92437618
TGCCGAG12600.023.92437620
Produced by FastQC (version 0.11.5)