FastQCFastQC Report
Mon 6 Jun 2016
ERR1378461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796951
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTATGATCTCGTATGCCGTC46990.5896221976006053Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTATGATCTCGTATGCC27800.34882947634170736Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT23670.2970069678060508Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC11360.1425432680302804Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5800.040.73951743
ATGCCGT6000.039.38153544
TGCCGTC6250.037.80627445
ATCGGAA5250.026.14063327
AGATCGG5400.025.83113525
CCGTCTA9200.025.67716428
TCTCGTA9350.025.50585438
GATCGGA5400.025.41450526
CTCGTAT9300.025.40106439
CGTCTAT9350.025.26523429
TCGTATG9400.025.13084240
ACCGTCT9400.025.13084227
TCGGAAG5750.025.04134828
GTATGCC9450.025.00257942
AGACCGT9550.024.97169725
GACCGTC9600.024.84163926
CGTATGC9750.024.2317541
ATCTCGT9750.024.22870837
ATGCCGA15950.023.83809919
AATGCCG16150.023.5428918
Produced by FastQC (version 0.11.5)