FastQCFastQC Report
Mon 6 Jun 2016
ERR1378460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945566
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTGTATCTCGTATGCC41810.44216902892024457Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTGTATCTCGTATGCCGTC34280.36253418587385755Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13750.14541555005150353Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3750.037.80355545
TATGCCG3850.036.82164443
ATGCCGT3850.036.82164444
CGGAAGA7650.023.5478151
TCTCGTA7850.022.64184841
CTCGTAT7900.022.49854742
CGTACTG7950.022.35822732
TCGTATG8050.022.08282343
GACCGTA8200.021.9509629
ACCGTAC8250.021.81792630
AGACCGT8300.021.68649328
TATCTCG8200.021.67542839
CCGTACT8250.021.54520231
CGTATGC8300.021.41767744
ATCTCGT8550.020.7881340
GAGACCG8700.020.68831827
GTATGCC8600.020.67054745
AGATCGG3000.020.24762225
ATCGGAA2950.019.82922727
CCGAGAC9250.019.45717225
Produced by FastQC (version 0.11.5)