FastQCFastQC Report
Mon 6 Jun 2016
ERR1378459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818154
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATGTATCTCGTATGCC23420.2862541770864654Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATGTATCTCGTATGCCGTC20620.25203079126912536Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10380.1268709802799962Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2700.036.66681343
CGAACCG250.002107344335.99794424
ATGCCGT3100.031.93561444
TGCCGTC3100.031.93561445
GATCGGA2400.024.3736126
ATCGGAA2450.023.87618627
CGGAAGA5500.022.5110971
CGTATGC5600.022.09830544
TCGGAAG2800.021.6951928
AGACCGT5850.021.53723128
CTCGTAT5750.021.52182842
TCGTATG5800.021.33629443
AGATCGG2850.021.31457125
TATCTCG5850.021.15393339
TCTCGTA5900.020.97594541
CCGTCAT5900.020.97338131
ATCTCGT5950.020.79840340
GACCGTC6100.020.6545629
CGTCATG6100.020.6545632
GAGACCG6000.020.62382327