FastQCFastQC Report
Mon 6 Jun 2016
ERR1378458.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378458.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102437
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTAGTATCTCGTATGCC21010.19057778358309818Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTAGTATCTCGTATGCCGTC20400.18504458758187545Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12480.11320374769714732Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2450.036.73595443
ATGCCGT2850.031.5800344
TGCCGTC2850.031.5800345
AGATCGG2800.023.301225
GATCGGA2950.022.11639226
ATCGGAA3050.021.39126427
CGTCTAG4300.020.92810229
CGTATGC4500.020.49791341
TATCTCG4500.020.49791336
CTCGTAT4450.020.2226639
CCGTCTA4500.019.99796528
TCGGAAG3400.019.8509228
TCTCGTA4650.019.35286738
AGACCGT4650.019.35286725
TCGTATG4700.019.14698640
AATGCCG8400.019.0159218
TGCCGAG8400.019.0159220
ATCTCGT4750.018.9454437
GACCGTC4750.018.9454426
ACCGTCT4800.018.7480927
Produced by FastQC (version 0.11.5)