FastQCFastQC Report
Mon 6 Jun 2016
ERR1378457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765627
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTCTATCTCGTATGCC27030.3530439757218593Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTCTATCTCGTATGCCGTC24790.323786909291339Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12440.16248120821235407Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2600.038.9492643
ATGCCGT2750.036.8247644
TGCCGTC2950.034.32816345
CGGAAGA6250.023.7875541
CGTAGTC6100.023.23578332
CTCGTAT6100.022.8699542
CCGTAGT6250.022.67812531
ACCGTAG6250.022.67812530
TCGTATG6200.022.50401943
GTATGCC6400.022.50401745
TCTCGTA6200.022.50107841
GACCGTA6250.022.31815329
CGTATGC6300.022.14681244
TATCTCG6350.021.96668639
GAGACCG6400.021.7950727
ATCTCGT6450.021.62611840
AGACCGT6550.021.29594828
ACGCCCG654.2406854E-420.76615512
GTCTATC7100.019.96183436
CGAGACC7250.019.85912926
Produced by FastQC (version 0.11.5)