FastQCFastQC Report
Mon 6 Jun 2016
ERR1378456.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378456.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955220
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCC31720.3320700990347773Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCCGTC30020.3142731517346789Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15720.1645694185632629Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3400.036.40167643
TGCCGTC3650.033.90841345
ATGCCGT3800.033.16209844
CGGTATC6800.022.4993232
GAACCGC602.46934E-422.49696411
CCGGTAT6850.022.33509331
ACCGGTA6900.022.17324330
CTCGTAT6800.022.1696142
GACCGGT7050.022.0206129
AGACCGG6950.022.01372328
TCTCGTA6850.022.00778641
CGTATGC6900.021.85059744
TCGTATG6900.021.85059743
AGATCGG3300.021.81523725
GAGACCG7100.021.54638727
GATCGGA3350.021.48963726
CGGGATT1700.021.17361514
ACGGGAT1601.8189894E-1221.09090413
CCTTCGT1501.4551915E-1121.019171
TATCTCG7400.020.97799739
Produced by FastQC (version 0.11.5)