FastQCFastQC Report
Mon 6 Jun 2016
ERR1378454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909098
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGATCTATCTCGTATGCCGTC32110.35320724498348915Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGATCTATCTCGTATGCC29330.32262748350562864Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT14170.15586878422348305Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4200.035.9046243
ATGCCGT4400.034.27259444
TGCCGTC4500.033.5109845
AGACCGT7250.021.09991525
CGGAAGA7100.020.941941
TATCTCG7250.020.78962136
ATCGGAA2850.020.5228827
GACCGTG7500.020.39658426
ACCGTGA7650.019.9966527
CGTATGC7550.019.96464241
GAGACCG7800.019.90051324
GATCGGA2950.019.82718726
TCTCGTA7750.019.7386338
AATGCCG10800.019.58005518
ATCTCGT7600.019.53620337
GTATGCC7750.019.45370742
TCGTATG7750.019.44835740
CTCGTAT7800.019.32368739
ATGCCGA10950.019.31183419
GAGCGGT11150.019.1809083
Produced by FastQC (version 0.11.5)