FastQCFastQC Report
Mon 6 Jun 2016
ERR1378453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435334
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATCAATCTCGTATGCCGTC23050.5294785153468371Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATCAATCTCGTATGCC20120.4621738710966752Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13020.2990807058488425Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC4790.11003045937142517Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2550.038.82868643
ATGCCGT2750.036.0047844
TGCCGTC2800.035.3618445
ATCGGAA2400.029.06301927
TCGGAAG2500.028.80051428
AGATCGG2500.027.89729325
GATCGGA2550.027.35028626
AATGCCG6950.024.2782218
CCGAGAT3000.023.99767322
GAATGCC7200.023.74769617
CTCGTAT4200.023.57184839
CGTATGC4250.023.29453541
GACCGTC4250.023.29185926
GTATGCC4300.023.02631242
TCTCGTA4300.023.02102338
TGCCGAG7400.022.80184220
TCGTATG4350.022.75902740
GAGACCG4400.022.49781824
AGACCGT4400.022.49781825
GCCGAGA7450.022.04484221
Produced by FastQC (version 0.11.5)