FastQCFastQC Report
Mon 6 Jun 2016
ERR1378452.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378452.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881418
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTACTATCTCGTATGCCGTC39610.4493895064543724Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTACTATCTCGTATGCC35820.40639061149193684Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT22470.25493012395934733Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10020.11368045581097731Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4900.036.738443
ATGCCGT5050.035.2015744
TGCCGTC5200.034.18614245
CGGCTAA855.456968E-1231.76070217
CCGGCTA1058.54925E-1125.71104416
ATCGGAA4300.023.54354327
AGATCGG4500.022.99710325
TCGGAAG4600.022.4984428
AACGGGT500.002265853322.49716412
GATCGGA4650.021.77144826
AATGCCG13200.021.64499918
CGTATGC8350.021.55661441
CGTGTAC8250.021.54518329
CTCGTAT8400.021.42708639
TCTCGTA8450.021.30029938
TATCTCG8650.021.06790436
TCGTATG8650.021.06790440
AGACCGT8450.021.0328525
ATGCCGA13600.021.0083819
ATCTCGT8750.020.82712737
Produced by FastQC (version 0.11.5)