FastQCFastQC Report
Mon 6 Jun 2016
ERR1378451.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378451.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202773
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACTACTATCTCGTATGCCGTC21490.17867045568864615Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTACTATCTCGTATGCC17590.14624538462369874Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2600.031.16081443
GAACCGC554.1686853E-628.63324211
TACGGTT406.1582524E-428.1312944
ATGCCGT3200.025.31816144
TGCCGTC3300.024.55094345
TCTCGTA4600.023.4786341
CGACTAC4700.022.97812832
CTCGTAT4650.022.74229242
CGTATGC4750.022.74192644
CCGACTA4650.022.74134831
ACCGACT4650.022.74040230
GACCGAC4750.022.26165629
TCGTATG5050.021.3909243
TCGGAAG2550.021.17504328
GATCGGA2550.021.17416226
TATCTCG5000.021.14857139
AGACCGA5000.021.14857128
GAGACCG5150.020.9694627
ATCTCGT5050.020.93918240
AGATCGG2900.020.17021625
Produced by FastQC (version 0.11.5)