FastQCFastQC Report
Mon 6 Jun 2016
ERR1378450.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378450.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938003
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCATACTATCTCGTATGCCGTC19500.20788846091110585Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATACTATCTCGTATGCC12020.1281445794949483Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11210.11950921265710236Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3050.036.8875243
ATGCCGT3200.036.5647544
TGCCGTC3650.030.82381645
CGCATAC4350.025.34233729
CGTATGC4450.025.2811141
CTCGTAT4500.024.99754139
CCGCATA4500.024.49759128
TCTCGTA4600.024.45411738
TATCTCG4700.024.41249536
TCGTATG4900.023.87520440
ATCTCGT4750.023.68188337
AGACCGC4700.023.45514125
ACCGCAT4800.023.43519627
TACTATC4950.023.17953933
ACGCCCG500.002265595622.49778712
GACCGCA4900.022.49778726
GAGACCG5000.022.04783224
GATCGGA2750.021.27063426
AGAGCGG8250.021.0091322
GAGCGGT8300.020.8814583
Produced by FastQC (version 0.11.5)