FastQCFastQC Report
Mon 6 Jun 2016
ERR1378449.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378449.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071162
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGTATATCTCGTATGCC32600.30434238705256533Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGTATATCTCGTATGCCGTC29610.27642877547933925Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13920.1299523321402365Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4050.037.23272743
ATGCCGT4500.033.00931544
TGCCGTC4600.032.2917245
AGATCGG3950.022.21316125
GATCGGA3950.022.21316126
CTCGTAT7300.021.57639542
CGCTGTA7350.021.42961532
GACCGCT7400.021.28482229
TCTCGTA7400.021.28482241
CGTATGC7450.021.14690644
TCGTATG7450.021.14690643
CGGAAGA8100.021.124961
ACCGCTG7550.020.86194430
CCGCTGT7600.020.72469531
TATCTCG7600.020.7227639
ATCGGAA4300.020.40701927
AGACCGC7750.020.32262228
GAGACCG7850.020.06373627
AATGCCG11900.020.04018818
ATGCCGA11900.020.04018819
Produced by FastQC (version 0.11.5)