FastQCFastQC Report
Mon 6 Jun 2016
ERR1378448.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378448.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608130
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTACATCTCGTATGCCGTC42690.7019880617631098Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTACATCTCGTATGCC38810.6381859141959778Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15830.2603061845329124Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7260.11938236890138622Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4800.037.98589343
ATGCCGT4850.037.59428844
TGCCGTC5050.036.10540845
ACCGCGT250.00210506436.004417
AGATCGG3100.026.12578625
CGGAAGA7800.025.1219541
ATCGGAA3250.024.91998127
GATCGGA3300.024.54240626
TCGGAAG3600.022.49720428
CGTACTA9250.021.40274632
TCGTATG9250.021.17171943
TCTCGTA9300.021.04750441
GACCGTA9200.021.02999529
GTATGCC9350.020.94528445
CCGTACT9300.020.80386731
GAAGAGC9750.020.7751923
GTACTAC9550.020.7304133
AGACCGT9350.020.69261628
ACCGTAC9350.020.69261630
CGTATGC9600.020.63431444
Produced by FastQC (version 0.11.5)