FastQCFastQC Report
Mon 6 Jun 2016
ERR1378445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738680
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTATAATCTCGTATGCCGTC29880.4045053338387393Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTATAATCTCGTATGCC28780.38961390588617534Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15840.21443656251692206Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3600.039.37683543
ATGCCGT3750.037.8043244
TGCCGTC3800.037.30689645
GATCGGA3150.029.28311726
ATCGGAA3150.028.56889327
AGATCGG3450.026.73675725
CGAGATC4000.024.74780323
TCGGAAG3650.024.65534628
GAGATCG3950.023.92192824
CCGAGAT4700.021.54064422
AATGCCG10850.021.35755318
TGCCGAG11250.021.19811820
ATGCCGA11150.021.1864619
TCGTATG7000.020.56960340
CGTATGC6900.020.54304541
CTCGTAT6900.020.54165539
CGGAAGA6800.020.5393031
AGTTCGT550.00393879520.4513448
CGAGTAT6950.020.39387329
GCCGAGA11700.020.38280721
Produced by FastQC (version 0.11.5)