FastQCFastQC Report
Mon 6 Jun 2016
ERR1378444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728503
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGATAATCTCGTATGCCGTC27440.3766628277440175Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGATAATCTCGTATGCC24380.3346588826676074Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15320.21029426097078527Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3550.038.04099343
ATGCCGT3900.035.78129244
TGCCGTC4050.033.9003245
ATCGGAA2700.024.1648627
TCTCGTA5600.024.10534138
CGTATGC5750.023.87433641
CTCGTAT5750.023.8677839
AGACCGT5850.023.84437225
ACCGTAG5850.023.84437227
CCGTAGA5900.023.64230228
TCGTATG5750.023.47811940
GACCGTA5950.023.44362626
ATCTCGT5950.023.06550237
CGTAGAT6100.022.86714429
GATCGGA2900.022.4983226
GAGACCG6300.022.14120324
AGATCGG2850.022.10361125
TCGGAAG3300.021.81655128
TGCCGAG9800.021.80959720
GTATGCC6200.021.77854342
Produced by FastQC (version 0.11.5)