FastQCFastQC Report
Mon 6 Jun 2016
ERR1378443.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378443.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374415
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATATGAATCTCGTATGCCGTC29660.7921691171560968Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATGAATCTCGTATGCC20870.5574028818289866Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT17380.4641908043214081Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7410.1979087376307039Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3850.038.00935443
TGCCGTC4000.037.1468345
ATGCCGT4100.036.2408144
AGCGTCT300.00513817430.00561141
CGTATGC5600.026.12095541
CCGATAT5550.025.94040128
ACCGATA5550.025.94040127
CTCGTAT5650.025.87942539
TCGTATG5650.025.87942540
CGATATG5650.025.87942529
TCTCGTA5750.025.4293538
AGACCGA5750.025.03812825
GTATGCC5950.024.58771542
ATCTCGT5950.024.5745837
AATCTCG5950.024.5745836
GACCGAT5900.024.40156426
GAGACCG5950.024.19650824
ATGAATC6200.023.5836733
CGAGACC6200.023.22084223
TCGGAAG3200.023.19816628
Produced by FastQC (version 0.11.5)