FastQCFastQC Report
Mon 6 Jun 2016
ERR1378442.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378442.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871842
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTATAGAATCTCGTATGCCGTC28230.32379720178656224Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATAGAATCTCGTATGCC24460.28055542173926007Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT16060.18420768900787068Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3650.036.37515343
TGCCGTC4000.033.1923245
ATGCCGT4050.032.78254344
ATCGGAA3800.026.05193527
GATCGGA3850.025.71359626
TCGGAAG4000.025.31182328
AGATCGG4050.024.44098725
ATGCCGA10100.022.71955919
CCGTATA5750.022.30375128
AATGCCG10450.022.17389718
TGCCGAG10450.021.95861620
TCTCGTA6100.021.76171138
GACCGTA5900.021.73670626
ACCGTAT5900.021.73670627
CGTATGC6200.021.41071741
CGTATAG6100.021.3928729
GAGATCG4750.021.31277324
ATCTCGT6450.020.9296737
CTCGTAT6450.020.58084339
TCGTATG6450.020.58084340
Produced by FastQC (version 0.11.5)