FastQCFastQC Report
Mon 6 Jun 2016
ERR1378441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896214
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCA651.4031684E-831.15318932
CGTATGC602.4184192E-729.99936944
CGTGCAA300.005147190829.99936936
GAGCGGT654.8698894E-727.693276
ACCGGAC709.305422E-725.71374530
TCTCGTA709.305422E-725.71374541
CTCGTAT751.6972608E-623.99949542
GACCGGA751.6972608E-623.99949529
CCGGACC751.6972608E-623.99949531
AGCGGTT802.9726452E-622.500787
ATCTCGT802.9740859E-622.49952540
GCGGTTC802.9740859E-622.4995258
CGGTTCA855.0335184E-621.174849
AGAGCGG908.245446E-620.0006945
CGTGAGC1251.7573257E-819.8084241
CACCGCG706.982815E-419.2853098
ACCGCGC706.9854374E-419.2842319
AGACCGG951.3155424E-518.94696828
TCGTATG951.3155424E-518.94696843
AATGCCG951.3161754E-518.94591121
Produced by FastQC (version 0.11.5)