FastQCFastQC Report
Mon 6 Jun 2016
ERR1378440.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378440.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654168
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA651.4011675E-831.15479941
CGCGTGA300.005144885730.00091742
GACCGGG702.8812792E-828.92945729
CGGGACA702.8812792E-828.92945732
ATCTCGT702.883462E-828.92724640
AGCGGTT803.3887773E-928.125867
CCGGGAC755.6297722E-827.00082431
CTCGTAT755.6297722E-827.00082442
GAGCGGT755.6297722E-827.0008246
CGTATGC801.0520307E-725.31327444
GCGGTTC801.0520307E-725.3132748
TATCTCG801.0528129E-725.31133839
CGGAAGA751.6908252E-624.0080741
AGACCGG903.2814933E-722.50068928
TCGTATG903.2814933E-722.50068943
ATCGTCA500.002263173222.5006875
CCGAGAC1056.1007086E-821.4275925
ACCGGGA955.5225973E-721.31644230
GTATGCC955.5225973E-721.31644245
CGGTTCA955.5266537E-721.3148149
Produced by FastQC (version 0.11.5)