FastQCFastQC Report
Mon 6 Jun 2016
ERR1378438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077735
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA801.05392246E-725.31184829
TCGTATG901.1912562E-824.99935543
CTCGTAT851.8938954E-723.82291642
TCTCGTA851.8938954E-723.82291641
AGACCGG955.532438E-721.31523928
AACCGCT550.00393696920.45401831
ACCGGAA1009.056075E-720.24947730
CGTATGC1009.056075E-720.24947744
CCGGAAC1009.056075E-720.24947731
CGGAACA1051.4457546E-619.28521732
ATCTCGT1102.2562708E-618.40861740
CGGAAGA1253.8262624E-718.0028761
AGAGCGG1406.466507E-817.6805785
AGCGGTT1459.65847E-817.070117
GCGGTTC1358.579318E-716.6670118
GAGACCG1358.5834836E-716.66623727
GAGCGGT1401.2530345E-616.0732526
AAGAGCG1552.0619154E-715.9702954
CGGTTCA1502.5738282E-614.9996149
CCGAGAC1351.5817546E-514.99961425
Produced by FastQC (version 0.11.5)