FastQCFastQC Report
Mon 6 Jun 2016
ERR1378433.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378433.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510573
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCAA403.4518234E-739.3769732
AAGAGCG458.6706314E-735.008614
CTCGTAT458.6905857E-734.9983242
GACCGGT501.9779945E-631.50157729
GAGCGGT501.9779945E-631.5015776
CCGGTCA501.9779945E-631.50157731
CGGAAGA554.147856E-628.6462141
ACCGGTC554.1573585E-628.63779630
GAGACCG554.1573585E-628.63779627
CGTATGC554.160529E-628.63499344
CGGTTCA554.160529E-628.6349939
CCGAGAC554.160529E-628.63499325
TCGTATG554.160529E-628.63499343
AGAGCGG608.176716E-626.2513125
GCGGTTC608.1829385E-626.2487438
TCTCGTA651.5221607E-524.22960941
TGCCGAG651.5221607E-524.22960923
ATCTCGT702.7001914E-522.49892440
AATGCCG702.7001914E-522.49892421
ATGCCCG500.00226352922.49892212
Produced by FastQC (version 0.11.5)