FastQCFastQC Report
Mon 6 Jun 2016
ERR1378432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706499
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT700.038.5751886
CGTATGC700.038.5697344
GCGGTTC751.8189894E-1235.9984178
CGGTTCA751.8189894E-1235.9984179
CTCGTAT803.6379788E-1233.74851242
AATGCCG803.6379788E-1233.74851221
AGACCGT855.456968E-1231.76330828
AGCGGTT901.2732926E-1130.0029267
AGAGCGG901.2732926E-1130.0029265
CGTGCAA901.2732926E-1129.99867832
GACCGTG952.3646862E-1128.419829
GAGACCG952.3646862E-1128.419827
TCTCGTA952.3646862E-1128.419841
TCGTATG952.3646862E-1128.419843
ACCGTGC952.3646862E-1128.419830
TGCCGAG952.3646862E-1128.419823
CGGAAGA1004.5474735E-1127.0160221
AAGAGCG1004.5474735E-1127.0045454
CCGAGAC1004.5474735E-1126.99881225
ATGCCGA1004.5474735E-1126.99881222
Produced by FastQC (version 0.11.5)