FastQCFastQC Report
Mon 6 Jun 2016
ERR1378431.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378431.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977364
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA300.005147372429.99942227
CGGACAA450.001226561524.99951732
CCGGACA450.001226561524.99951731
TCGTATG551.3677022E-424.54623643
CTCGTAT500.002263950222.50071742
TCTCGTA500.002263950222.50071741
ACCGGAC500.002264623322.49956530
ATCGCAC550.00393189920.4583365
ACGCCAT550.003937729620.45310417
CGCCACC1656.002665E-1119.0944485
TCGCACC600.00650285318.7534756
GACCGGA600.006510541318.7496429
GAGACCG750.001110205517.99965127
AGCGCTC750.001110205517.9996517
CGTATAA750.001110586817.99873210
CACGCCC1104.6391713E-516.36248411
GAGCGGT850.002561626515.8852986
CCACGCC1907.998096E-915.39365210
CGCTCCA1056.0389424E-414.9989429
GCGCTCC900.00375514614.9989428
Produced by FastQC (version 0.11.5)