FastQCFastQC Report
Mon 6 Jun 2016
ERR1378430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071270
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTA501.0822987E-940.4995833
ACCGTCG552.748493E-936.817830
CTCGTAT606.4319465E-933.74965342
TCGTATG606.4319465E-933.74965343
CGTCGTT606.4319465E-933.74965332
CCGTCGT651.4035322E-831.15352231
TCGTTAA702.8861905E-828.9282734
CGTATGC702.8861905E-828.9282744
AGACCGT702.8861905E-828.9282728
CGTTAAT803.3960532E-928.1247135
AATGCCG755.6415956E-826.99845921
ATCTCGT801.05375875E-725.31223940
CGGTTCA901.19162E-824.9985739
GACCGTC851.8935862E-723.82328229
GAGACCG1003.6441634E-822.49976727
CGAGGAC500.002265260822.49871618
GCGGTTC1151.596054E-719.56418
AAGAGCG1051.4429406E-619.2891164
TGCCGAG1051.4455254E-619.28551323
AGCGGTT1051.4461712E-619.2846157
Produced by FastQC (version 0.11.5)