FastQCFastQC Report
Mon 6 Jun 2016
ERR1378429.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378429.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971475
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT458.698935E-734.9995642
CCGTAGT501.981396E-631.49960331
TCTCGTA501.981396E-631.49960341
GACCGTA608.190642E-626.2496729
ACCGTAG608.190642E-626.2496730
TCGTATG702.702699E-522.49971843
GAGCGGT702.7037739E-522.498566
CGTATGC754.6021363E-520.99973744
AGCGGTT754.603962E-520.9986557
ATCTCGT807.562189E-519.68725240
AGACCGT951.3156314E-518.9471328
CATCTCG851.2043132E-418.52917939
GCGGTTC851.2047886E-418.5282278
CGTAGTT901.8654809E-417.4997832
AAGAGCG952.8124268E-416.583864
GAGACCG952.819061E-416.5787427
ATGCCGA1004.166612E-415.74980222
CGGTTCA1004.168244E-415.748999
CACCGCG900.00375496314.999048
AAAGCGC900.00375496314.999045
Produced by FastQC (version 0.11.5)