FastQCFastQC Report
Mon 6 Jun 2016
ERR1378427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968774
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT700.038.5708142
GACCGCC852.0372681E-1029.1171829
AGACCGC852.0372681E-1029.1171828
ACCGCCA952.3646862E-1128.42059730
TCTCGTA952.3646862E-1128.42059741
GCGGTTC957.421477E-1026.0522148
CGTATGC1058.54925E-1125.71387344
ATCTCGT1001.3442332E-924.74960140
AGCGGTT1001.3442332E-924.7496017
TCGTATG1101.5097612E-1024.54506143
CGGAAGA1052.3574103E-923.5783521
CGGTTCA1052.3665052E-923.571059
GAGCGGT1104.0490704E-922.5008016
CCGAGAC1104.0508894E-922.4996425
GAGACCG1156.7666406E-921.52139527
ATGCCGA1156.7666406E-921.52139522
CCGCCAT1156.7666406E-921.52139531
AATGCCG1201.1048542E-820.6246721
TGCCGAG1201.1048542E-820.6246723
AGAGCGG1302.7697752E-819.0391395
Produced by FastQC (version 0.11.5)