FastQCFastQC Report
Mon 6 Jun 2016
ERR1378425.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378425.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109654
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC855.456968E-1231.76515244
TATCTCG855.456968E-1231.76515239
CGGTTGT1004.5474735E-1127.00037832
GACCGGT1004.5474735E-1127.00037829
GAGCGGT1004.5474735E-1127.0003786
TCGTATG1004.5474735E-1127.00037843
CGGAAGA1105.456968E-1226.593681
CGGTTCA1200.026.2491869
ACCGGTT1058.54925E-1125.71464730
CTCGTAT1058.54925E-1125.71464742
TCTCGTA1101.5097612E-1024.545841
AGCGGTT1101.5279511E-1024.5446937
GCGGTTC1152.6557245E-1023.4775318
CCGGTTG1204.5110937E-1022.50031731
ATCTCGT1257.530616E-1021.60030240
AGAGCGG1257.530616E-1021.6003025
AGACCGG1301.2278178E-920.76952428
CGCGCCC654.238991E-420.76858711
GAGACCG1351.9626896E-920.00028227
AATGCCG1351.9645086E-919.9993821
Produced by FastQC (version 0.11.5)