FastQCFastQC Report
Mon 6 Jun 2016
ERR1378424.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378424.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954017
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT601.5643309E-1037.49847429
ACCGTTG708.1308826E-1032.1415530
TATCTCG751.7025741E-930.00034739
ACGGGTT300.005147850629.99877713
CCGTTGG702.8863724E-828.92739331
TCTCGTA803.3942342E-928.12532641
CTCGTAT856.4774213E-926.47089442
CGGTTCA856.4810592E-926.4695079
AGACCGT856.4810592E-926.46950728
ATCTCGT901.1903467E-825.0002940
GAGCGGT901.1910743E-824.9989816
CGTTGGT851.8936953E-723.82255732
CGTATGC952.1142114E-823.68448844
GCGGTTC952.1153028E-823.6832478
AGCGGTT952.1153028E-823.6832477
GAGACCG1052.3665052E-923.57046927
CCTTCGT1056.0772436E-821.4378091
AGAGCGG1151.5953083E-719.564425
CGTTGGA1503.43789E-1019.4992055
CTTCGTT1302.7621354E-819.0436742
Produced by FastQC (version 0.11.5)