FastQCFastQC Report
Mon 6 Jun 2016
ERR1378423.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378423.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044365
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC551.1274824E-732.7274830
CTCGTAT501.9812906E-631.50020242
CGATCGT501.9812906E-631.50020232
GACCGAT602.418492E-730.00019329
GCGGTTC755.6403223E-826.9988788
GATCGTT608.190202E-626.25016833
TCTCGTA608.190202E-626.25016841
CGTATGC651.5229376E-524.23208244
CCGATCG651.5235024E-524.23092331
CGTTATC651.5235024E-524.23092336
TCGTATG651.5235024E-524.23092343
TATCTCG702.7025546E-522.50014339
GCGCCCG500.00226501822.49906712
CGCCCGG702.7035545E-522.49906513
ACCGCGC654.2390605E-420.7683689
ATGCCGA908.24961E-620.00012822
ATCGTTA807.561779E-519.68762634
AGCGGTT1051.4458838E-619.2849147
CCGCGCC706.984836E-419.28491410
CACCGCG706.984836E-419.2849148
Produced by FastQC (version 0.11.5)