FastQCFastQC Report
Mon 6 Jun 2016
ERR1378420.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378420.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087250
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC606.4283086E-933.751528
CCGGATC606.4337655E-933.74841331
CGGATCT606.4337655E-933.74841332
CGTATGC702.8860086E-828.92854344
ACCGGAT702.8872819E-828.92721230
CTCGTAT801.053686E-725.31247342
TCGTATG851.893477E-723.82350543
GACCGGA851.8943138E-723.82240929
TCTCGTA903.286641E-722.49997741
AGCGGTT955.528764E-721.316757
ATGCCGA955.533657E-721.31478722
TATCTCG1009.0540925E-720.24997939
CGGTTCA1009.058076E-720.2490469
ACGCCAT807.562575E-519.68747940
ATCTCGT1202.496381E-718.7499840
AGACCGG1102.2567656E-618.40822628
CGGAAGA1153.4377772E-617.6143471
GAGCGGT1153.4468394E-617.6094886
CCGAGAC1305.784714E-717.30687725
AATGCCG1205.1748975E-616.87420721
Produced by FastQC (version 0.11.5)