FastQCFastQC Report
Mon 6 Jun 2016
ERR1378418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011839
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT501.9814615E-631.4996929
AGACCGA501.9814615E-631.4996928
ACCGATG554.164609E-628.63608230
CGTATGC651.5236337E-524.23052844
TCTCGTA651.5236337E-524.23052841
GGGCGAC751.6974591E-623.99976228
CGGAAGA802.9693256E-622.5042251
CCGATGC702.7027869E-522.49977731
GAGACCG702.7027869E-522.49977727
GAGCGGT702.7027869E-522.4997776
AGCGGTT855.0316594E-621.176267
ATGCCGA855.0316594E-621.1762622
GCGGTTC754.6022855E-520.999798
TCGTATG754.6022855E-520.9997943
CGATGCT908.250348E-619.99980432
TATCTCG807.5624324E-519.68730539
CCGAGAC951.3156892E-518.94718225
CGGTTCA851.2043519E-418.5292269
AGAGCGG1002.0464155E-517.9998235
TGCCGAG1053.1120908E-517.14268723
Produced by FastQC (version 0.11.5)