FastQCFastQC Report
Mon 6 Jun 2016
ERR1378417.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378417.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005539
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA702.1827873E-1135.3572629
CGTACCT754.7293724E-1133.0001132
ACCGTAC754.7293724E-1133.0001130
TCTCGTA855.456968E-1231.76481241
GCGGTTC801.0004442E-1030.9376038
AGCGGTT852.0372681E-1029.1177447
CCGTACC852.0372681E-1029.11774431
AGACCGT852.0372681E-1029.11774428
GAGCGGT903.947207E-1027.5028276
CGTATGC903.947207E-1027.50009244
TCGTATG903.947207E-1027.50009243
AATCTCG1058.54925E-1125.7143739
CTCGTAT1001.3442332E-924.75008242
GAGACCG1001.3442332E-924.75008227
CGGTTCA1001.3460522E-924.7476229
ATCTCGT1104.0508894E-922.50007640
CCGAGAC1156.7702786E-921.52074225
CGGAAGA1056.080154E-821.4371721
AGAGCGG1201.103399E-820.627125
TGCCGAG1201.1053999E-820.62404323
Produced by FastQC (version 0.11.5)