FastQCFastQC Report
Mon 6 Jun 2016
ERR1378416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566247
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCTTATCTCGTATGCC7100.12538697776765265Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC950.042.63266844
CTCGTAT950.042.63266842
TATCTCG950.042.62890239
ACCGAAC1000.040.5010330
TCGTATG1000.040.5010343
CGAACCT1050.038.57241432
CCGAACC1050.038.57241431
GACCGAA1100.036.8191229
TCTCGTA1100.036.8191241
GAGACCG1250.034.2008727
AGACCGA1250.032.40082628
ATCTCGT1250.032.39796440
CCGAGAC1300.031.15189225
AACGGGT300.00514665829.99811712
GAGCGGT1400.028.9344226
AGCGGTT1450.027.9342147
GTATGCC1500.027.0006945
CGGTTCA1500.026.9983049
AGAGCGG1550.026.1343155
CGGAAGA1550.024.680231
Produced by FastQC (version 0.11.5)